Calpain activity in fast, slow, transforming, and regenerating skeletal muscles of rat

Calpain activity in fast, slow, transforming, and regenerating skeletal muscles of rat.

Fiber-type transitions in adult skeletal muscle induced by chronic low-frequency stimulation (CLFS) encompass coordinated exchanges of myofibrillar protein isoforms. CLFS-induced elevations in cytosolic Ca(2+) could activate proteases, especially calpains, the major Ca(2+)-regulated cytosolic proteases. Calpain activity determined by a fluorogenic substrate in the presence of unaltered endogeno...

Regenerating slow motor axons navigate toward their own regions within adult skeletal muscles of the rat

The aerobic maternal exercise increases PDK4 gene expression in slow and fast twitch muscles of rat male offspring.

Recent studies indicate that environment can modify skeletal muscle gene expression and transcriptional factors in offspring genes. Therefore, exercising can lead to changes in the gene expression of the future generations. One of the important metabolic factors is PDK4, which is known as the main factor in Pyruvate Dehydrogenase Complex (PDC). This study examined the effects of maternal exerci...

Effect of progressive resistance exercise on β1 integrin and vinculin protein levels in slow-and fast-twitch skeletal muscles of male rats

Introduction: Skeletal muscle is a flexible and ever changing tissue and the role of costameric proteins in its response to different stimuli is not well defined. The aim of this study was to investigate the effect of progressive resistance exercise on β1 integrin and vinculin proteins in fast and slow twitch skeletal muscles of male rats. Methods: Twelve male Wistar rats (weight: 298±5.2 gr...

Activity-dependent plasticity of transmitter release from nerve terminals in rat fast and slow muscles.

Neuromuscular junctions (NMJs) on fast and slow muscle fibers display different transmitter release characteristics that appear well adapted to the different patterns of nerve impulses that they transmit in vivo. Here, we ask whether the release properties of such NMJs, termed fast and slow, can be transformed by chronic nerve stimulation. In young adult rats, nerve impulse conduction in the sc...